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Short Communication • Rev. Soc. Bras. Med. Trop. 51 (01) • Jan-Feb 2018 • https://doi.org/10.1590/0037-8682-0026-2017   copy

Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION.

This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients.

METHODS.

A total of 134 CoNS strains were characterized using four different methods.

RESULTS.

The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1.00). The kappa index of Vitek 2® Compact analysis was 0.85 (very good) and that of the conventional method was 0.63 (moderate).

CONCLUSIONS

. MALDI-TOF MS provided rapid and accurate results for the identification of CoNS (134; 100%).

Keywords:
Coagulase-negative staphylococci; MALDI-TOF; tuf gene

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