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Major Article • Rev. Soc. Bras. Med. Trop. 51 (02) • Mar-Apr 2018 • https://doi.org/10.1590/0037-8682-0404-2017   copy

Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year.

METHODS:

In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR).

RESULTS:

All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods.

CONCLUSIONS:

The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.

Keywords:
Dengue virus; Arbovirus; Flavivirus; Viral diagnostic; Flow cytometry

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