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Major Article • Rev. Soc. Bras. Med. Trop. 51 (03) • May-Jun 2018 • https://doi.org/10.1590/0037-8682-0372-2017   copy

Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

This study evaluated the performance of the IS6110-TaqMan® assay in different types of biological samples and tissues for laboratory diagnosis of extrapulmonary tuberculosis.

METHODS:

143 biological samples and tissues from patients with suspected extrapulmonary tuberculosis from the health services of Recife/Pernambuco/Brazil were evaluated with the IS6110-TaqMan® assay.

RESULTS:

The sensitivities of the IS6110-TaqMan® assay calculated for blood, urine, both blood and urine samples, tissue biopsies, extrapulmonary body fluid samples, and all samples from patients calculated together were 55.9%, 33.3%, 68.8%, 43.8%, 29.6%, and 73.7%, respectively, and the specificities were 80%, 100%, 78.6%, 100%, 100%, and 84.2%, respectively.

CONCLUSIONS

The accuracy of qPCR was high in various clinical sample types. The analysis of more than one type of clinical sample collected from the same patient with extrapulmonary tuberculosis enhances the diagnostic power of the IS6110-TaqMan® assay when compared with the use of only one clinical sample.

Keywords:
Mycobacterium tuberculosis complex; IS6110-TaqMan® assay; Molecular diagnosis; Extrapulmonary tuberculosis

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