Natural history of experimental arthritis induced by <i>Paracoccidioides brasiliensis</i> in wistar rats

Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. This study aimed to evaluate the natural history of Paracoccidioides brasiliensis-induced experimental arthritis of the knee joints in Wistar rats.

METHODS:

Rats were randomly allocated to either an absolute control group, or 15-day, 45-day, or 90-day experimental (fungus-inoculated) groups.

RESULTS:

Experimental groups developed classic signs of articular PCM. Titers of anti-gp43 were observed to increase during the interval from 15 to 45 days post-inoculation.

CONCLUSIONS:

Articular arthritic lesions were induced and progressed during the study period in all experimental groups.

Keywords:
Arthritis; Paracoccidioidomycosis; Paracoccidioides brasiliensis

Authorship

Eduardo Alexandre Loth

Laboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.Universidade Estadual do Oeste do ParanáBrazilCascavel, PR, BrazilLaboratório Experimental de Microbiologia e Análises Clínicas, Ensino, Pesquisa e Extensão, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brasil.

Vanessa Cecatto

Rodrigo Daniel Genske

Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, São José dos Campos, SP, Brasil.Universidade do Vale do ParaíbaBrazilSão José dos Campos, SP, BrazilInstituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, São José dos Campos, SP, Brasil.

Maricília Silva Costa

Rinaldo Ferreira Gandra

Cleverson Marcelo Pilatti

Corresponding author: Vanessa Cecatto. e-mail:vane_cecatto@hotmail.com

Conflict of Interest: The authors declare that there is no conflict of interest.

SCIMAGO INSTITUTIONS RANKINGS

Figures

Figures (3)

Thumbnail

FIGURE 1:
Joint edema dynamics throughout the experimental interval. Multiple comparisons performed using an ANOVA (Tukey post-hoc) demonstrated the presence of a statistically significant difference in experimental group edema between the beginning and end of the experimental interval (p=0.0001 15-day Group, p=0.005 45-day Group, and p=0.005 90-day Group).

Thumbnail

FIGURE 2:
Titers of anti-gp43 antibodies against Pb. Peak antibody titers were observed at 45 days. Multiple comparisons performed using an ANOVA (Tukey post-hoc) demonstrated a statistically significant difference between the day 45 group and the other groups. *(p = 0.002).

Thumbnail

FIGURE 3:
Wistar rat knee joint sagittal section photomicrographs. Sections A, B, C, E, and F were stained with Hematoxylin and Eosin; sections D, G, and H were stained using the Grocott’s stain. A: control group with preserved anatomical aspects: articular cartilage (Ca), cavity (*), meniscus (Me), joint capsule (Cp), femur (Fe), and tibia (Ti). Inset shows details of the synovial membrane, including synoviocytes (arrow) and sub-intima exhibiting a predominance of adipose (Ad) cells. B: Day 15 experimental group: synovitis with granulomatous inflammation and edema at the synovial membrane (Ms) of the perimeniscal region, as well as sub-intimal neovascularization (Vs). Inset shows details of the intimal layer (In), which exhibited thickening. C and D: Day 45 experimental group: synovial membrane with pannus containing granulomas featuring fungi (tip of the arrow). E to H: Day 90 experimental group. E: Synovial membrane exhibiting mature granulomas (delimited by a dashed line). F: Mature granulomas (delimited by a dashed line) located in joint-adjacent regions and perimuscularly (Mm), with areas of lytic necrosis. G and H: Presence of Pb (tip of the arrow) in the subchondral bone of the femur.

FIGURE 1: Joint edema dynamics throughout the experimental interval. Multiple comparisons performed using an ANOVA (Tukey post-hoc) demonstrated the presence of a statistically significant difference in experimental group edema between the beginning and end of the experimental interval (p=0.0001 15-day Group, p=0.005 45-day Group, and p=0.005 90-day Group).

FIGURE 2: Titers of anti-gp43 antibodies against Pb. Peak antibody titers were observed at 45 days. Multiple comparisons performed using an ANOVA (Tukey post-hoc) demonstrated a statistically significant difference between the day 45 group and the other groups. *(p = 0.002).

FIGURE 3: Wistar rat knee joint sagittal section photomicrographs. Sections A, B, C, E, and F were stained with Hematoxylin and Eosin; sections D, G, and H were stained using the Grocott’s stain. A: control group with preserved anatomical aspects: articular cartilage (Ca), cavity (*), meniscus (Me), joint capsule (Cp), femur (Fe), and tibia (Ti). Inset shows details of the synovial membrane, including synoviocytes (arrow) and sub-intima exhibiting a predominance of adipose (Ad) cells. B: Day 15 experimental group: synovitis with granulomatous inflammation and edema at the synovial membrane (Ms) of the perimeniscal region, as well as sub-intimal neovascularization (Vs). Inset shows details of the intimal layer (In), which exhibited thickening. C and D: Day 45 experimental group: synovial membrane with pannus containing granulomas featuring fungi (tip of the arrow). E to H: Day 90 experimental group. E: Synovial membrane exhibiting mature granulomas (delimited by a dashed line). F: Mature granulomas (delimited by a dashed line) located in joint-adjacent regions and perimuscularly (Mm), with areas of lytic necrosis. G and H: Presence of Pb (tip of the arrow) in the subchondral bone of the femur.

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Natural history of experimental arthritis induced by <i>Paracoccidioides brasiliensis</i> in wistar rats

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[1] Corresponding author: Vanessa Cecatto. e-mail:vane_cecatto@hotmail.com