Revista do Hospital das Clínicas
On-line version ISSN 1678-9903
FERNANDEZ, Solange Assuncion Villagra et al. Prevalence of antinuclear autoantibodies in the serum of normal blood dornors. Rev. Hosp. Clin. [online]. 2003, vol.58, n.6, pp. 315-319. ISSN 1678-9903. http://dx.doi.org/10.1590/S0041-87812003000600005.
OBJECTIVE:To examine the presence of serum antinuclear autoantibodies in a healthy population. METHODS: Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion. RESULTS: In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40. CONCLUSION: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.
Keywords : Autoantibodies; Healthy population; Rheumatic diseases; Antinuclear antibodies; Autoimmunity.