Print version ISSN 0044-5967
HANDA, Lucia; SAMPAIO, Paulo de Tarso B. and QUISEN, Regina Caetano. Culture in vitro of rosewood (Aniba rosaeodora Ducke) embryos´seeds and buds explants. Acta Amaz. [online]. 2005, vol.35, n.1, pp. 29-33. ISSN 0044-5967. http://dx.doi.org/10.1590/S0044-59672005000100005.
This study deals with the establishment in vitro of Aniba rosaeodora Ducke explants, free from fungical and endogenous contaminations and phenolic oxidation. Bud explants and embryos' seeds from many maturation stages were used in this trial. The explants were disinfected with Ampicilin antibiotic, Streptomicine Sulphate (Agrimicina), etanol (70%), sodium hipoclorite in many concentrations and exposure time acording to the type of explant. For the phenolic oxidation control, the immersion on ascorbic acid and PVP (Polyvinilpirrolidone) in culture medium were used. The explants were inoculated in MS medium. The statistical design was the completely randomized and the treatments and repetitions varied according to the type of explant adopted. After 45 days, 100% of survival and 53% of germination in embryos were observed, which had been desinfested by sodium hipoclorite (50%) for 10 minutes and inoculated in MS medium, supplemented with 20 ml of coconut water. Satisfactory results (51% of survival) were observed in bud´s explants, which had been treated by immersion in 300 mg.l-1 of Agrimicina and 25% when submitted in 500 mg.l-1 on vacuum pre-treatment.
Keywords : rosewood; Aniba rosaeodora Ducke; tissue culture; embryoculture; buds.