On-line version ISSN 1809-4392
NUNOMURA, Rita de Cássia Saraiva et al. In vitro studies of the anthelmintic activity of Picrolemma sprucei Hook. f. (Simaroubaceae). Acta Amaz. [online]. 2006, vol.36, n.3, pp. 327-330. ISSN 1809-4392. http://dx.doi.org/10.1590/S0044-59672006000300006.
1300 ppm (1.3 g / L), water and ethanol extracts prepared from stems or roots of Picrolemma sprucei Hook. f. were lethal (85-90 % mortality) in vitro to Haemonchus contortus (Barber Pole Worm) larvae, a gastrointestinal nematode parasite found in domestic and wild ruminants. Neosergeolide and isobrucein B were isolated in 0.0083 and 0.0070 % yield from dry, ground P. sprucei stems (0.89 kg). Neosergeolide, isobrucein B and the anthelmintic drug standard levamisole all caused comparable mortality rates (68-77 %) in vitro to H. contortus at similar concentrations (81-86 ppm). The anthelmintic activity of P. sprucei infusions (teas), alcohol extracts, and neosergeolide and isobrucein B, has therefore been demonstrated for the first time.
Keywords : Picrolemma pseudocoffea Ducke; Haemonchus contortus; neosergeolide; isobrucein B; caferana.