versión impresa ISSN 0044-5967
DIAS, Danielle de Carla et al. Macrophagic migration time in matrinxã, Brycon amazonicus, through inoculation of yeast, Saccharomyces cerevisiae, technique. Acta Amaz. [online]. 2011, vol.41, n.3, pp. 421-424. ISSN 0044-5967. http://dx.doi.org/10.1590/S0044-59672011000300013.
In aquaculture, analysis of activation and increased macrophages migration are used in order to verify the ability of the nonspecific immune fish exposed to a challenge. This study aimed to determine the time of macrophages migration in matrinxã, Brycon amazonicus, through the technique of yeast Saccharomyces cerevisiae inoculation, verifying possible changes in hematological parameters. Thirty animals with average weight of 101.55 ± 24.50 g and average length of 19.75 ± 1.72 cm were employed. The experimental inoculation periods were 2, 4, 8 and 12 hours. Thereafter, animals were anesthetized and blood was withdrawn through a caudal puncture for the determination of total erythrocytes number, differential and total leukocyte counts and total thrombocytes count, hematocrit, hemoglobin concentration and calculation of the erythrocytes index. The results for the phagocytic capacity were not significantly different between experimental periods. In the phagocytic index, the period of 2 hours presented the highest rate of phagocytized cells, indicating that 2 hours of incubation was sufficient for the macrophages migration in B. amazonicus. The number of erythrocyte was the only parameter that presented significant difference among periods.
Palabras llave : Phagocytosis; Inoculation; Hematology.