Genetic diversity of <i>Gossypium barbadense</i> from the central Brazilian Amazon

HOFFMANN, Lúcia Vieira; CARDOSO, Kálita Cristina Moreira; ROCHA, Antônio Sabino Neto da Costa; OLIVEIRA, Aryanny Irene Domingues de; ABREU, Aluana Gonçalves; PEREIRA, Caio César de Oliveira; MALAFAIA, Guilherme; MENEZES, Ivandilson Pessoa Pinto de

doi:10.1590/1809-4392201603313

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Agronomy and Forestry • Acta Amaz. 48 (1) • Jan-Mar 2018 • https://doi.org/10.1590/1809-4392201603313 copy

Genetic diversity of Gossypium barbadense from the central Brazilian Amazon

Diversidade genética de Gossypium barbadense coletado na Amazônia central

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The Amazon Basin is a center of diversity of Gossypium barbadense and the strategy for conservation of this genetic resource depends on the knowledge of the diversity maintained in Amazonas State. During two expeditions, in 2012 and 2014, plants were collected in ten municipalities in the state of Amazonas, in the central Brazilian Amazon region. The molecular diversity was estimated by SSR markers for 50 samples collected in 2012. The morphological diversity of 24 plants collected in 2014 was assessed ex situ and compared to that of 50 plants of the same and other cotton varieties from other Brazilian states. Most of plants evaluated in situ in Amazonas had purple petioles and veins (82%), associated to medicinal use, and kidney seeds (78%). The ex situ morphological analysies showed that G. barbadense plants from the Amazonas state: i) presented higher similarity to cotton plants from other northern Brazilian states, and ii) were grouped separately from those of other northern Brazilian states by descriptor analysis. Both the molecular (H=0.41) and morphological (H=0.38±0.02) diversity among the collected plants was considered intermediary. Our study indicates the distinctiveness of Amazon cottons, and contributes to demonstrate the discrimination power of multicategorical traits.

KEYWORDS:
conservation; genetic resources; cotton tree; phenotypic characterization

N	Multicategorical traits	Description
1	Stem color	1) Green	2) Purplish	3) Purple
2	Stem hairiness	1) Glabrous	2) Litle hairy	3) Hairy	4) Very hairy
3	Number of lobes	1) Three	2) Five	3) Seven
4	Leaf color	1) Green	2) Purplish	3) Purple
5	Rib color	1) Colorless	2) Green	3) Purplish	4) Purple
6	Petiole color	1) Colorless	2) Green	3) Purplish	4) Purple
7	Nectary on leaves	1) Absence	2) Mainrib	3) Mainand lateral rib
8	Leaf shape	1) Palmate	2) Semi-digitate	3) Digitate	4) Lanceolate
9	Leaf size	1) Small	2) Medium	3) Big
10	Leaf hairiness (upper surface)	1) Glabrous	2) Little hairy	3) Hairy	4) Very hairy
11	Leaf hairiness (lower surface)	1) Glabrous	2) Little hairy	3) Hairy	4) Very hairy
12	Bracteole dentation	1) Less than 7	2) Between 7 and 12	3) More than 12
13	Bracteole length	1) Small	2) Medium	3) Long
14	Bracteole width	1) Narrow	2) Normal	3) Large
15	Nectaries at the base of the bracteole	1) Absence	2) Incipient	3) Present
16	Internal nectary of the bracteole	1) Absence	2) Incipient	3) Present
17	Corolla colour	1) Creamy	2) Light yellow	3) Dark yellow	4) Yellow and redapex
18	Petal spot	1) Absente	2) Incipient	3) Low	4) Medium	5)Strong
19	Overlapping petals	1) Little overlapping	2) Overlapping	3) Very overlapping
20	Petal length	1) Small	2) Medium	3) Long
21	Stigma position in relation to anthers	1) Below	2) Same height	3) Above	4) Farabove
22	Position of staminal column	1) Short	2) Medium	3) Long
23	Pollen colour	1) Creamy	2) Light yellow	3) Dark yellow	4) Orange

Locus	Allele1	G. barbadense (n = 50)	G. hirsutum
Locus	Allele1	G. barbadense (n = 50)	Delta	FM966	Loren	M315
CIR246	146	0.00	1.00	1.00	0.00	0.00
CIR246	156	1.00	0.00	0.00	1.00	1.00
DC20027	200	0.86	0.00	1.00	1.00	1.00
	202	0.02	1.00	0.00	0.00	0.00
	206	0.12	0.00	0.00	0.00	0.00
CIR316M	192	0.50	0.00	0.00	0.00	0.00
	198	0.00	1.00	1.00	1.00	0.00
	201	0.00	1.00	1.00	1.00	1.00
	204	0.50	0.00	0.00	0.00	0.00
	210	0.00	0.00	0.00	0.00	1.00
BNL3279	106	0.37	0.00	0.00	0.00	0.00
	114	0.49	1.00	1.00	0.00	1.00
	116	0.00	0.00	0.00	1.00	0.00
	124	0.03	1.00	1.00	1.00	1.00
	130	0.10	0.00	0.00	0.00	0.00
	144	0.01	0.00	0.00	0.00	0.00
BNL3661	181	0.10	0.00	0.00	0.00	0.00
	185	0.40	0.00	0.00	0.00	0.50
	189	0.50	0.00	0.00	0.00	0.50
	191	0.00	0.50	0.50	0.50	0.00
	193	0.00	0.50	0.50	0.50	0.00

Species	N	P (%)	H (SE)
G. barbadense	46	93.7	0.35 (± 0,03)
G. hirsutum r. marie galante	23	57.1	0.23 (± 0,03)
G. hirsutum var. punctatum	4	28.6	0.15 (± 0,03)
Unknown cotton	3	54	0.29 (± 0,04)
G. hirsutum latifolium var. australiana	1	-	-
Total	77	100	0.40 (± 0,03)

	G. barbadense	G. hirsutum r. marie galante	Unknown cotton	G. hirsutum latifolium var. australiana	G. hirsutum var. punctatum
G. barbadense	0.00
G. hirsutum r. marie galante	0.71	0.00
Unknown cotton	0.74	0.64	0.00
G. hirsutum latifolium var. australiana	0.72	0.50	0.69	0.00
G. hirsutum var. punctatum	0.73	0.43	0.66	0.38	0.00

Instituto Nacional de Pesquisas da Amazônia Av. André Araujo, 2936 Aleixo, 69060-001 Manaus AM Brasil, Tel.: +55 92 3643-3030, Fax: +55 92 643-3223 - Manaus - AM - Brazil
E-mail: acta@inpa.gov.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Corresponding author: ivan.menezes@ifgoiano.edu.br