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Arquivos Brasileiros de Cardiologia
On-line version ISSN 1678-4170
RODRIGUES, Alfredo José; EVORA, Paulo Roberto Barbosa; MARUO, Ayako and SCHAFF, Hartzell V.. Amiodarone causes endothelium-dependent vasodilation in canine coronary arteries. Arq. Bras. Cardiol. [online]. 2005, vol.84, n.3, pp. 251-255. ISSN 1678-4170. http://dx.doi.org/10.1590/S0066-782X2005000300011.
OBJECTIVE: To assess the vasodilating effects of amiodarone on canine coronary arteries by using solutions of amiodarone dissolved in polysorbate 80 or water. METHODS: Rings of coronary arteries, with or without intact endothelium, were immersed in Krebs solution and connected to a transducer for measuring the isometric force promoted by a vascular contraction. The arteries were exposed to increasing concentrations of polysorbate 80, amiodarone dissolved in water, amiodarone dissolved in polysorbate 80, and a commercial presentation of amiodarone (Cordarone). The experiments were conducted in the presence of the following enzymatic blockers: only indomethacin, Nw-nitro-L-arginine associated with indomethacin, and only Nw-nitro-L-arginine. RESULTS: Polysorbate 80 caused a small degree of nonendothelium-dependent relaxation. Cordarone, amiodarone dissolved in water, and amiodarone dissolved in polysorbate 80 caused endothelium-dependent relaxation, which was greater for amiodarone dissolved in polysorbate and for Cordarone. Only the association of indomethacin and Nw-nitro-L-arginine could eliminate the endothelium-dependent relaxation caused by amiodarone dissolved in polysorbate 80. CONCLUSION: The results obtained indicate that vasodilation promoted by amiodarone in canine coronary arteries is mainly caused by stimulation of the release of nitric oxide and cyclooxygenase-dependent relaxing endothelial factors.
Keywords : endothelium; amiodarone; nitric oxide; cyclooxygenase; coronary circulation.