Memórias do Instituto Oswaldo Cruz
versão impressa ISSN 0074-0276
BARRAL-NETTO, Manoel e BARRAL, A.. A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding. Mem. Inst. Oswaldo Cruz [online]. 1986, vol.81, n.3, pp. 343-345. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02761986000300012.
We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
Palavras-chave : concanavalin; mononuclear cells; visceral leishmaniasis.