Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276
On-line version ISSN 1678-8060
FIGUEIREDO, Luiz Tadeu Moraes et al. Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies. Mem. Inst. Oswaldo Cruz [online]. 1989, vol.84, n.3, pp.303-307. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02761989000300003.
This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
Keywords : Mayaro; alphavirus; arbovirus; enzyme immunoassay.