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Memórias do Instituto Oswaldo Cruz
On-line version ISSN 1678-8060
MORGADO, M. G. et al. Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera. Mem. Inst. Oswaldo Cruz [online]. 1989, vol.84, n.3, pp.309-314. ISSN 1678-8060. http://dx.doi.org/10.1590/S0074-02761989000300004.
Soluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumentar leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzy antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumentar leishmaniasis. On the other hand, 10 polypeptidesspecifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera teste and may be good candidates for specific immunodiagnosis of Chagas' disease.
Keywords : western blot; Trypanosoma cruzi; antigens; specifity; cross-reactivity.