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Memórias do Instituto Oswaldo Cruz
On-line version ISSN 1678-8060
CASTRO, Solange L. de; SOEIRO, Maria Nazaré C. and MEIRELLES, Maria de Nazareth Leal de. Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells. Mem. Inst. Oswaldo Cruz [online]. 1992, vol.87, n.2, pp. 209-215. ISSN 1678-8060. http://dx.doi.org/10.1590/S0074-02761992000200007.
Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed.
Keywords : Trypanosoma cruzi; phenothiazines; calcium antagonists; parasite-host cell interactions.