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Memórias do Instituto Oswaldo Cruz

versión impresa ISSN 0074-0276versión On-line ISSN 1678-8060


COUTO-FERNANDEZ, José Carlos; MORGADO, Mariza Gonçalves; SANTOS, Jairo Ivo dos  y  GALVAO-CASTRO, Bernardo. Molecular and biological diversity of HIV-1 in Brazil. Mem. Inst. Oswaldo Cruz [online]. 1992, vol.87, n.2, pp.249-255. ISSN 0074-0276.

To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.

Palabras clave : Brazilian HIV; virulence; restriction analysis; viral diversity.

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