Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276
LEAO, Ana Maria dos A. Carneiro; CARVALHO JUNIOR, Luiz B. and MALAGUENO, Elizabeth. The use of ferromagnetic dacron as solid-phase in enzyme immunoassays. Mem. Inst. Oswaldo Cruz [online]. 1994, vol.89, n.2, pp. 189-193. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02761994000200012.
Ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. Human serum albumin (HSA) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used anti-HSA rabbit sera. Peroxidase, o-phenylenediamine (OPD) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. Best results were observed when particles of 63-100 µm (diameter) and 10 µg of immobilized antigen were used. Positive reactions were detected until dilutions of1:51200 of immune sera. Its reproducibility was similar to standard ELISA. Disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
Keywords : magnetic ELISA; ferromagnetic dacron; human serum albumin.