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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060

Abstract

ARAUJO, Aureci Maria et al. The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague. Mem. Inst. Oswaldo Cruz [online]. 1996, vol.91, n.2, pp.195-198. ISSN 0074-0276.  https://doi.org/10.1590/S0074-02761996000200014.

Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3 ¼g of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3% w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 1:12,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.

Keywords : polyvinyl alcohol; glutaraldehyde; Yersinia pestis; ELISA; Brazil.

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