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Memórias do Instituto Oswaldo Cruz
On-line version ISSN 1678-8060
Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction. Mem. Inst. Oswaldo Cruz [online]. 1997, vol.92, n.5, pp. 595-600. ISSN 1678-8060. http://dx.doi.org/10.1590/S0074-02761997000500006.
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas
Keywords : dengue; diagnosis; virological surveillance; polymerase chain reaction.