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Memórias do Instituto Oswaldo Cruz

versión impresa ISSN 0074-0276versión On-line ISSN 1678-8060

Resumen

CASTILLO, Marta C de; SESMA, Fernando; NADER, Olga M de  y  HOLGADO, Aida P de Ruiz. Properties of ß-lactamase from Neisseria gonorrhoeae . Mem. Inst. Oswaldo Cruz [online]. 1998, vol.93, n.2, pp.237-241. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02761998000200020.

ß-lactamase activity was studied in Neisseria gonorrhoeae strains. Optimum temperature was found to be 37°C. The enzyme was inactivated at temperatures higher than 60°C, but remained active during storage at low temperatures (4°C, -30°C and -70°C) for two months. Enzyme activity was observed within a pH range of 5.8-8.0, while the optimum pH was 7.0-7.2. Addition of Ni2+, Fe2+, Fe3+, Mn2+ and p-chloromercurybenzoate to the reaction buffer exerted a negative effect upon the activity, whereas Hg2+ and ethylene diamine tetra-acetic acid produced complete inhibition. These results would indicate the presence of -SH groups at the catalytic site of the enzyme.

Palabras clave : Neisseria gonorrhoeae; b-lactamase; enzyme activity.

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