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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276

Abstract

GOMES, Yara M et al. Partial Protection of Mice against Trypanosoma cruzi after Immunizing with the TcY 72 Antigenic Preparation. Mem. Inst. Oswaldo Cruz [online]. 1999, vol.94, n.2, pp. 167-172. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02761999000200007.

A 72 kDa Trypanosoma cruzi glycoprotein recognized by the 164C11 monoclonal antibody (IgM isotype) was purified by preparative electrophoresis. The antigenic preparation obtained, named TcY 72, was used to immunize C57Bl/10 mice. The following results were observed after immunization: (1) induction of higher titres of IgG than IgM antibodies, as evaluated by indirect immunofluorescence; (2) significant DTH after injection of epimastigotes in mice footpads; (3) peak parasitemia in immunized mice was significantly reduced and animals were negative by 13 days post-infection, although the mice still succumb to infection; (4) the phenotypic analysis of spleen cell populations showed a decrease in the CD4/CD8 ratio in immunized mice. Taken as a whole, these findings indicate that TcY 72 is immunogenic and potentially important for protective immunity.

Keywords : Trypanosoma cruzi; immunization; TcY antigenic preparation; Chagas' disease.

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