SciELO - Scientific Electronic Library Online

 
vol.94 issue4Positive IgG Western Blot for Borrelia burgdorferi in ColombiaSensitivity of Lymph Node Aspiration in Localized Cutaneous Leishmaniasis Due to Leishmania (Viannia) braziliensis author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276

Abstract

ROMERO, Gustavo Adolfo Sierra; SAMPAIO, Raimunda Nonata Ribeiro; MACEDO, Vanize de Oliveira  and  MARSDEN, Philip Davis. Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission . Mem. Inst. Oswaldo Cruz [online]. 1999, vol.94, n.4, pp. 505-508. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02761999000400014.

Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.

Keywords : Leishmania (Viannia) braziliensis; culture sensitivity; fungal contamination; vacuum aspiratory technique.

        · text in English     · pdf in English