Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276
On-line version ISSN 1678-8060
MEIRELLES, Maria de Nazareth Leal de et al. Trypanosoma cruzi-cardiomyocytes: new contributions regarding a better understanding of this interaction. Mem. Inst. Oswaldo Cruz [online]. 1999, vol.94, suppl.1, pp.149-152. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02761999000700017.
The present paper summarizes new approaches regarding the progress done to the understanding of the interaction of Trypanosoma cruzi-cardiomyocytes. Mannose receptors localized at the surface of heart muscle cell are involved in binding and uptake of the parasite. One of the most striking events in the parasite-heart muscle cells interaction is the disruption of the actin cytoskeleton. We have investigated the regulation of the actin mRNA during the cytopathology induced in myocardial cells by the parasite. T. cruzi invasion increases calcium resting levels in cardiomyocytes. We have previously shown that Ca2+ ATPase of the sarcoplasmic reticulum (SERCA) is involved in the invasion of T. cruzi in cardiomyocytes. Treating the cells with thapsigargin, a drug that binds to all SERCA ATPases and causes depletion of intracellular calcium stores, we found a 75% inhibition in the T. cruzi-cardiomyocytes invasion.
Keywords : Trypanosoma cruzi; cardiomyocytes; calcium; SERCA ATPases; mRNA regulation.