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Papers presented at the International Symposium on Molecular Evoluation of Pathogenic Microorganisms Vectors and Reservoirs - 13/15 August 2000, Rio de Janeiro • Mem. Inst. Oswaldo Cruz 96 (3) • Apr 2001 • https://doi.org/10.1590/S0074-02762001000300026   copy

Genetic markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) obtained by the double stringency polymerase chain reaction technique

Authorship SCIMAGO INSTITUTIONS RANKINGS

Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.

Biomphalaria; DNA polymorphism; PCR

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