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Memórias do Instituto Oswaldo Cruz
On-line version ISSN 1678-8060
BELLO, Martha Darce; GONZALEZ, Alcides; BARNABE, Christian and LARROUY, Georges. First characterization of Candida albicans by random amplified polymorphic DNA method in Nicaragua and comparison of the diagnosis methods for vaginal candidiasis in Nicaraguan women. Mem. Inst. Oswaldo Cruz [online]. 2002, vol.97, n.7, pp.985-989. ISSN 1678-8060. http://dx.doi.org/10.1590/S0074-02762002000700010.
A total of 106 women with vaginitis in Nicaragua were studied. The positive rate for the identification of Candida species was 41% (44 positive cultures out of 106 women with vaginitis). The sensitivity of microscopic examination of wet mount with the potassium hydroxide (KOH) was 61% and 70% with Gram's stain when using the culture of vaginal fluid as gold standard for diagnosis of candidiasis. Among the 44 positives cultures, isolated species of yeast from vaginal swabs were C. albicans (59%), C. tropicalis (23%), C. glabrata (14%) and C. krusei (4%). This study reports the first characterization of 26 C. albicans stocks from Nicaragua by the random amplified polymorphic DNA method. The genetic analysis in this small C. albicans population showed the existence of linkage disequilibrium, which is consistent with the hypothesis that C. albicans undergoes a clonal propagation.
Keywords : vulvovaginal candidiasis; diagnosis; Candida albicans; molecular typing; random amplified polymorphic DNA; population structure; Nicaragua.