Memórias do Instituto Oswaldo Cruz
versão impressa ISSN 0074-0276
versão On-line ISSN 1678-8060
ANTAS, Paulo RZ et al. Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens. Mem. Inst. Oswaldo Cruz [online]. 2002, vol.97, n.8, pp.1097-1099. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762002000800005.
The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease.
Palavras-chave : activation markers; CD69; CD25; flow cytometry.