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vol.97 suppl.1Freshwater snails and Schistosomiasis mansoni in the state of Rio de Janeiro, Brazil: III - Baixadas MesoregionGenetic markers between Biomphalaria glabrata snails susceptible and resistant to Schistosoma mansoni infection author indexsubject indexarticles search
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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060


VIDIGAL, Teofânia HDA; MONTRESOR, Lângia C; SIMPSON, Andrew JG  and  CARVALHO, Omar S. Polymerase chain reaction and restriction fragment length polymorphism of cytocrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni. Mem. Inst. Oswaldo Cruz [online]. 2002, vol.97, suppl.1, pp.47-52. ISSN 0074-0276.

The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed to be the most informative once they were polymorphic patterns, corroborating with much morphological data. In addition, we performed COI digestion of B. straminea snails from Uruguay and Argentina.

Keywords : Biomphalaria; polymerase chain reaction; restriction fragment length polymorphism; cytochrome oxidase I.

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