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vol.97 suppl.1Partial molecular characterization of Sm8, a tegumental antigen of Schistosoma mansoniAntigenic community between Schistosoma mansoni and Biomphalaria glabrata: on the search of candidate antigens for vaccines índice de autoresíndice de materiabúsqueda de artículos
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Memórias do Instituto Oswaldo Cruz

versión impresa ISSN 0074-0276versión On-line ISSN 1678-8060

Resumen

VIDIGAL, THDA et al. A Multiplex-PCR approach to identification of the Brazilian intermediate hosts of Schistosoma mansoni. Mem. Inst. Oswaldo Cruz [online]. 2002, vol.97, suppl.1, pp.95-97. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762002000900019.

Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria, and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From the previous sequencing of the ITS2 region, one primer was designed to anchor in the 5.8S conserved region and three other species-specific primers in the 28S region, flanking the ITS2 region. These four primers were simultaneously used in the same reaction (Multiplex-PCR), under high stringency conditions. Amplification of the ITS2 region of Biomphalaria snails produced distinct profiles (between 280 and 350 bp) for B. glabrata, B. tenagophila and B. straminea. The present study demonstrates that Multiplex-PCR of ITS2-DNAr showed to be a promising auxiliary tool for the morphological identification of Biomphalaria snails, the intermediate hosts of S. mansoni.

Palabras clave : Biomphalaria; Multiplex-PCR; internal transcribed spacer 2.

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