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vol.98 suppl.1Techniques of DNA-studies on prehispanic ectoparasites (Pulex sp., Pulicidae, Siphonaptera) from animal mummies of the Chiribaya Culture, Southern PeruAnalysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach índice de autoresíndice de assuntospesquisa de artigos
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Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276versão On-line ISSN 1678-8060

Resumo

MARTINEZ, Elaine Machado et al. Random amplified polymorphic DNA analysis of DNA extracted from Trichuris trichiura (Linnaeus, 1771) eggs and its prospective application to paleoparasitological studies. Mem. Inst. Oswaldo Cruz [online]. 2003, vol.98, suppl.1, pp.59-62. ISSN 1678-8060.  https://doi.org/10.1590/S0074-02762003000900011.

Random amplified polymorphic DNA analysis was applied to DNAs extracted from Trichuris trichiura eggs recovered from human fecal samples. Four out of 6 primers tested displayed 18 distinct and well defined polymorphic patterns, ranging from 650 to 3200 base pairs. These results, upon retrieval and DNA sequencing of some of these bands from agarose gels, might help in establishing T. trichiura specific genetic markers, not available yet, and an important step to design primers to be used in molecular diagnosis approaches.

Palavras-chave : random amplified polymorphic DNA analysis; Trichuris trichiura; paleoparasitology.

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