Memórias do Instituto Oswaldo Cruz
versão impressa ISSN 0074-0276
ANTAS, Paulo RZ et al. Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals. Mem. Inst. Oswaldo Cruz [online]. 2004, vol.99, n.1, pp. 53-55. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762004000100009.
The production of interferon g (IFNg) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r2 = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.
Palavras-chave : tuberculosis (TB); T cell-responses; whole blood; PPD; latent TB infection; interferon g.