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Memórias do Instituto Oswaldo Cruz

versión impresa ISSN 0074-0276versión On-line ISSN 1678-8060

Resumen

TAVECHIO, Ana Terezinha; MARQUES, Lilian Regina M; ABE, Cecília Mari  y  GOMES, Tânia AT. Detection of cytotoxic necrotizing factor types 1 and 2 among fecal Escherichia coli isolates from brazilian children with and without diarrhea. Mem. Inst. Oswaldo Cruz [online]. 2004, vol.99, n.1, pp.81-83. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762004000100014.

The enteropathogenic role of cytotoxic necrotizing factor (CNF)-producing Escherichia coli was investigated by searching cnf genes among 2074 isolates from 200 children with and 200 without acute diarrhea in Brazil. Fourteen (7%) cases versus 10 (5%) control children carried at least one cnf positive isolate (P = 0.50) and most isolates expressed CNF type 1. DNA sequences of virulence factors of extraintestinal pathogenic E. coli (ExPEC) were detected in 78.6% of CNF1-producing isolates. Besides not being associated with human acute diarrhea, the CNF1-producing isolates here identified may represent potential ExPEC transitorily composing the normal intestinal flora.

Palabras clave : Escherichia coli ; cytotoxins; cytotoxic necrotizing factor; adhesins.

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