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Memórias do Instituto Oswaldo Cruz

versión impresa ISSN 0074-0276versión On-line ISSN 1678-8060

Resumen

ROSA, Florence Mara et al. Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni. Mem. Inst. Oswaldo Cruz [online]. 2004, vol.99, n.1, pp.85-87. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762004000100015.

Biomphalaria tenagophila population from Taim (state of Rio Grande do Sul, Brazil) is totally resistant toSchistosoma mansoni, and presents a molecular marker of 350 bp by polymerase chain reaction and restriction fragment length polymorphism of the entire rDNA internal transcriber spacer. The scope of this work was to determine the heritage pattern of this marker. A series of cross-breedings between B. tenagophila from Taim (resistant) and B. tenagophila from Joinville, state of Santa Catarina (susceptible) was carried out, and their descendants F1 and F2 were submitted to this technique. It was possible to demonstrate that the specific fragment from Taim is endowed with dominant character, since the obtained segregation was typically mendelian.

Palabras clave : Biomphalaria tenagophila; polymerase chain reaction and restriction fragment length polymorphism; dominant character; Taim, Rio Grande do Sul - Brazil.

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