SciELO - Scientific Electronic Library Online

vol.99 issue2Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNAAbnormal expression of CD54 in mixed reactions of mononuclear cells from hyper-IgE syndrome patients author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060


VIDIGAL, Teofânia HDA et al. Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae). Mem. Inst. Oswaldo Cruz [online]. 2004, vol.99, n.2, pp.153-158. ISSN 0074-0276.

The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the Biomphalaria species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that B. tenagophila represents a complex comprising B. tenagophila, B. occidentalis and B. t. guaibensis.

Keywords : Biomphalaria; snails; ribosomal DNA; internal transcribed spacer; polymerase chain reaction.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License