SciELO - Scientific Electronic Library Online

 
vol.99 número3The sexual behaviour of Panstrongylus megistus (Hemiptera: Reduviidae): an experimental studyVirulence markers and antimicrobial susceptibility of bacteria of the Bacteroides fragilis group isolated from stool of children with diarrhea in São Paulo, Brazil índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276versão On-line ISSN 1678-8060

Resumo

DINIZ-MENDES, Leonardo; DEVALLE, Sylvie  e  NIEL, Christian. Genomic characterization of a Brazilian TT Virus isolate closely related to SEN virus-F. Mem. Inst. Oswaldo Cruz [online]. 2004, vol.99, n.3, pp.301-306. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762004000300011.

SEN virus (SENV) is a circular, single stranded DNA virus that has been first characterized in the serum of a human immunodeficiency virus type 1 (HIV-1)-infected patient. Eight genotypes of SENV (A-H) have been identified and further recognized as variants of TT virus (TTV) in the family Circoviridae. Here we describe the first genomic characterization of a SENV isolate (5-A) from South America. Using 'universal' primers, able to amplify most, if not all, TTV/SENV genotypes, a segment of > 3 kb was amplified by polymerase chain reaction from the serum of an HIV-1 infected patient. The amplicon was cloned and a 3087-nucleotide sequence was determined, that showed a high (85%) homology with the sequence of the Italian isolate SENV-F. Proteins encoded by open reading frames (ORFs) 1 to 4 consisted of 758, 129, 276, and 267 amino acids, respectively. By phylogenetic analysis, isolate 5-A was classified into TTV genotype 19 (phylogenetic group 3), together with SENV-F and TTV isolate SAa-38.

Palavras-chave : hepatitis; human immunodeficiency virus type 1; nucleotide sequencing; open reading frames 1-4; SEN virus-F; TT virus.

        · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons