SciELO - Scientific Electronic Library Online

 
vol.100 issue7Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, BrazilMycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276

Abstract

SEQUEIRA, Patrícia Carvalho de; FONSECA, Leila de Souza; SILVA, Marlei Gomes da  and  SAAD, Maria Helena Féres. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Mem. Inst. Oswaldo Cruz [online]. 2005, vol.100, n.7, pp. 743-748. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762005000700012.

Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 Aids inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.

Keywords : IS1254; restriction fragment length polymorphism; Mycobacterium avium; MaDRE- polymerase chain reaction; nontuberculous mycobacteria; Aids.

        · text in English     · pdf in English