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Memórias do Instituto Oswaldo Cruz
On-line version ISSN 1678-8060
RODRIGUES, Flávia Guimarães; OLIVEIRA, Sabrina Barbosa; ROCHA, Bruno Coelho and MOREIRA, Luciano Andrade. Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element. Mem. Inst. Oswaldo Cruz [online]. 2006, vol.101, n.7, pp. 755-757. ISSN 1678-8060. http://dx.doi.org/10.1590/S0074-02762006000700008.
The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
Keywords : transgenic mosquitoes ; piggyBac ; malaria.