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Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276
On-line version ISSN 1678-8060
ENRIQUE PEREZ, J et al. Isolation and molecular identification of Leishmania ( Viannia ) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes. Mem. Inst. Oswaldo Cruz [online]. 2007, vol.102, n.5, pp.655-658. Epub July 27, 2007. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762007005000077.
Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.
Keywords : Leishmania peruviana; Lutzomyia peruensis; polymerase chain reaction-restriction fragment length polymorphism; Peru.