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Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276
On-line version ISSN 1678-8060
GUSMAO, Desiely Silva et al. First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association. Mem. Inst. Oswaldo Cruz [online]. 2007, vol.102, n.8, pp.919-924. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762007000800005.
We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
Keywords : Aedes aegypti; gut diverticulum; microbiota; Serratia sp.