SciELO - Scientific Electronic Library Online

vol.102 issue8Identification of hepatitis C virus subtype 2c by sequencing analysis in patients from Córdoba, ArgentinaFirst encounter of subclinical human Leishmania (Viannia) infection in State of Rio Grande do Sul, Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276On-line version ISSN 1678-8060


RODRIGUEZ, Islay et al. Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato. Mem. Inst. Oswaldo Cruz [online]. 2007, vol.102, n.8, pp.999-1002. ISSN 0074-0276.

The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.

Keywords : Borrelia; new medium; BSK-H medium; proteins.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License