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vol.103 número1Capillaria spp. eggs in Patagonian archaeological sites: statistical analysis of morphometric dataFirst visceral leishmaniasis focus in Argentina índice de autoresíndice de assuntospesquisa de artigos
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Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276versão On-line ISSN 1678-8060

Resumo

LELES, Daniela et al. Molecular paleoparasitological diagnosis of Ascaris sp. from coprolites: new scenery of ascariasis in pre-Colombian South America times. Mem. Inst. Oswaldo Cruz [online]. 2008, vol.103, n.1, pp.106-108.  Epub 31-Jan-2008. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762008005000004.

Paleoparasitological studies using microscopy showed that Ascarisand Trichuris trichiura are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.

Palavras-chave : Ascaris; molecular paleoparasitological diagnosis; paleodistribution.

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