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Memórias do Instituto Oswaldo Cruz

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Abstract

TAVECHIO, Ana T et al. Tracing lineage by phenotypic and genotypic markers in Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and Salmonella Typhimurium isolated in state of São Paulo, Brazil. Mem. Inst. Oswaldo Cruz [online]. 2009, vol.104, n.7, pp. 1042-1046. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762009000700019.

Fifty-three Salmonella 1,4,[5],12:i:- and 45 Salmonella Typhimurium strains were characterised using phage typing, plasmid profiles and pulsed-field gel electrophoresis (PFGE) for comparison. The majority of the strains were subdivided into definitive type (DT) 41 (22.6%) and DT 193 (18%) and the 60-MDa plasmid was detected in 94.3% and 84.4% of strains, respectively. Genetic diversity was observed among all strains and 90% presented a > 70% similarity through PFGE analysis. These results suggest a close relationship between Salmonella 1,4,[5],12:i:- and Salmonella Typhimurium at the serotype level.

Keywords : Salmonella phage typing; plasmids; pulsed-field gel electrophoresis.

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