SciELO - Scientific Electronic Library Online

 
vol.105 issue7Green propolis phenolic compounds act as vaccine adjuvants, improving humoral and cellular responses in mice inoculated with inactivated vaccinesTrypanosoma cruzi benznidazole susceptibility in vitro does not predict the therapeutic outcome of human Chagas disease author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276

Abstract

BEN, Renata; RODRIGUES, Rubens; AGOSTINI, Aventino Alfredo  and  GRAEFF-TEIXEIRA, Carlos. Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay. Mem. Inst. Oswaldo Cruz [online]. 2010, vol.105, n.7, pp. 914-917. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762010000700013.

Angiostrongylus costaricensis has a broad geographic distribution spanning from North to South America and the infections of vertebrates with this nematode can result in abdominal complications. Human infections are diagnosed by histological or serological methods because the isolation of larvae from feces is not feasible, as most parasites become trapped in intestinal tissues due to intense eosinophilic inflammation. Because A. costaricensis is difficult to maintain in the laboratory, an immunodiagnostic IgG enzyme-linked immunosorbent assay (ELISA) using antigens from the congeneric Angiostrongylus cantonensis species was evaluated against a panel of serum samples from patients who were histologically diagnosed with A. costaricensis infections. Sera from uninfected individuals and individuals infected with other parasites were used as controls. The sensitivity and specificity of the assay were estimated at 88.4% and 78.7%, respectively. Because the use of purified or cloned antigens has not been established as a reliable diagnostic tool, the use of heterologous antigens may provide a viable alternative for the development of an ELISA-based immunodetection system for the diagnosis of abdominal angiostrongyliasis.

Keywords : abdominal angiostrongyliasis; Angiostrongylus costaricensis; Angiostrongylus cantonensis; immunodiagnosis; eosinophilic gastroenteritis; helminths.

        · text in English     · pdf in English