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Memórias do Instituto Oswaldo Cruz

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MENDONCA, Marcos César Lima de et al. Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing. Mem. Inst. Oswaldo Cruz [online]. 2011, vol.106, n.4, pp.502-504. ISSN 0074-0276.

Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

Keywords : parvovirus B19; nucleotide sequencing; genotyping; HMA; SSCP.

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