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Memórias do Instituto Oswaldo Cruz

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Abstract

FERREIRA, João Leandro de Paula et al. Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples. Mem. Inst. Oswaldo Cruz [online]. 2011, vol.106, n.5, pp. 613-616. ISSN 0074-0276.  http://dx.doi.org/10.1590/S0074-02762011000500014.

In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.

Keywords : influenza; H1N1; haemagglutinin; polymorphism; Brazil.

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