Memórias do Instituto Oswaldo Cruz
Print version ISSN 0074-0276
FERREIRA, William Antunes et al. Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region. Mem. Inst. Oswaldo Cruz [online]. 2011, vol.106, n.5, pp.629-631. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762011000500018.
We report two ciprofloxacin and ofloxacin-resistant Neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the Alfredo da Matta Foundation (Manaus, state of Amazonas, Brazil). The gonococci displayed minimal inhibitory concentrations (> 32.00 µg/mL) and three mutations in the quinolone resistance-determining region (S91F and D95G in GyrA and S87R in ParC). Both isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing and the analysis showed that the ST225 which represented an emerging widespread multi-resistant clone that has also been associated with reduced susceptibility to ceftriaxone. We recommend continued surveillance of this pathogen to assess the efficacy of anti-gonococcal antibiotics in Brazil.
Keywords : Neisseria gonorrhoeae; fluroquinolone resistance; gyrA and parC; genotyping; MAST.