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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276


MELANDRI, Vanessa et al. Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil. Mem. Inst. Oswaldo Cruz [online]. 2012, vol.107, n.8, pp.1073-1075. ISSN 0074-0276.

In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.

Keywords : WNV; b-ELISA; PRNT; Brazil.

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