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vol.108 suppl.1When population and evolutionary genetics met behaviourMolecular analysis of an odorant-binding protein gene in two sympatric species of Lutzomyia longipalpis s.l. author indexsubject indexarticles search
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Memórias do Instituto Oswaldo Cruz

Print version ISSN 0074-0276

Abstract

GENTILE, Carla et al. Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference. Mem. Inst. Oswaldo Cruz [online]. 2013, vol.108, suppl.1, pp.80-87. ISSN 0074-0276.  https://doi.org/10.1590/0074-0276130471.

Mosquitoes are the culprits of some of the most important vector borne diseases. A species’ potential as a vector is directly dependent on their pattern of behaviour, which is known to change according to the female’s physiological status such as whether the female is virgin/mated and unfed/blood-fed. However, the molecular mechanism triggered by and/or responsible for such modulations in behaviour is poorly understood. Clock genes are known to be responsible for the control of circadian behaviour in several species. Here we investigate the impact mating and blood-feeding have upon the expression of these genes in the mosquito Aedes aegypti . We show that blood intake, but not insemination, is responsible for the down-regulation of clock genes. Using RNA interference, we observe a slight reduction in the evening activity peak in the fourth day after dstim injection. These data suggest that, as in Drosophila , clock gene expression, circadian behaviour and environmental light regimens are interconnected in Ae. aegypti .

Keywords : clock genes; blood-feeding; insemination; RNAi; behaviour; Aedes aegypti.

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