SciELO - Scientific Electronic Library Online

 
vol.109 número8Brazilian Angiostrongylus cantonensis haplotypes, ac8 and ac9, have two different biological and morphological profilesShe’s a femme fatale: low-density larval development produces good disease vectors índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276

Resumo

ROBLES-MURGUIA, Maricela; BLOEDOW, Nicholas; MURRAY, Leigh  e  RAMALHO-ORTIGAO, Marcelo. Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness. Mem. Inst. Oswaldo Cruz [online]. 2014, vol.109, n.8, pp.1064-1069. ISSN 0074-0276.  http://dx.doi.org/10.1590/0074-0276140382.

In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.

Palavras-chave : sandflies; PpChit1; peritrophic matrix; blood digestion.

        · texto em Inglês     · Inglês ( pdf )