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Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276

Resumo

BARBOSA, Joana; RODRIGUES, Acácio Gonçalves; PEREZ, Maria José  e  PINA-VAZ, Cidália. Evaluation of Giardia duodenalis viability after metronidazole treatment by flow cytometry. Mem. Inst. Oswaldo Cruz [online]. 2014, vol.109, n.8, pp.1078-1080.  Epub 21-Nov-2014. ISSN 0074-0276.  http://dx.doi.org/10.1590/0074-0276140267.

Giardia duodenalis (syn. lamblia; syn. intestinalis) susceptibility testing is not routinely performed because the classical culture methods are very time-consuming and laborious. We developed a novel flow cytometry (FC) assay to evaluate the susceptibility of G. duodenalis trophozoites to metronidazole (MTZ). Different concentrations of MTZ were added to cultures of trophozoites (10 5 /mL) and the cultures were incubated for different periods. The 50% inhibitory concentration was calculated and propidium iodide (PI) was used to quantify the number of dead cells. After treatment, PI-positive trophozoites increased with increasing drug concentration and exposure time. An excellent correlation was found between FC and the classical method. A novel, accurate and reliable method is now available to evaluate G. duodenalis viability.

Palavras-chave : Giardia duodenalis; flow cytometry; susceptibility.

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