Abel et al. (2001) |
Capture ELISA with PBMC culture supernatants |
Recombinant B13 protein |
48 h |
IFN-γ levels in CCHD > asymptomatic IL-10 undetectable in all clinical groups |
Guedes et al. (2012)Guedes PM, Gutierrez FR, Silva GK, Dellalibera-Joviliano R, Rodrigues GJ, Bendhack LM, Rassi Jr A, Rassi A, Schmidt A, Maciel BC, Marin Neto JA, Silva JS 2012. Deficient regulatory T-cell activity and low frequency of IL-17-producing T cells correlate with the extent of cardiomyopathy in human Chagas disease. PLoS Negl Trop Dis
6: e1630.
|
Capture ELISA with PBMC culture supernatants |
Trypomastigote lysate (Y strain) |
48 h |
IFN-γ levels in CCHD > asymptomatic IL-10 levels in asymptomatic > CCHD |
Gomes et al. (2003) |
FACS intracellular cytokine |
Epimastigote/trypomastigote lysate (Y strain) |
24 h for IL-10 6 days for IFN-γ |
CD3+IFN-γ+ in CCHD > asymptomatic CD14+IL-10+ in asymptomatic > CCHD |
Fonseca et al. (2005) |
ELISPOT |
HLA-A02.01-restricted cruzipain/ FL-160 peptides |
24 h |
IFN-γ+ cells in CCHD |
Gomes et al. (2014) |
CBA with PBMC culture supernatants |
Epimastigote/trypomastigote lysate (CL strain) |
22 h |
IFN-γ levels in CCHD = asymptomatic IL-10 levels in asymptomatic > CCHD |
Cuellar et al. (2009) |
FACS intracellular cytokine |
Recombinant KMP-11 T. cruziprotein |
12 h |
CD4+IFN-γ+ in CCHD > asymptomatic |
de Araújo et al. (2012) |
FACS intracellular cytokine |
Trypomastigote lysate (CL strain) |
22 h |
Treg IFN-γ+ in CCHD > asymptomatic Treg IL-10+ in asymptomatic = CCHD |
de Melo et al. (2012) |
Real time polymerase chain reaction |
Recombinant CRA/FRA proteins |
3 days |
IFN-γ expression in asymptomatic = CCHD IL-10 expression in asymptomatic = CCHD |
Albareda et al. (2006) |
FACS intracellular cytokine |
T. cruzi-infected dendritic cells (Brazil strain) |
6 h |
CD8+IFN-γ+ in asymptomatic > CCHD |
Diez et al. (2006) |
ELISPOT |
HLA-A02.01-restricted K1 peptide |
24 h |
IFN-γ+ lymphocytes in asymptomatic = CCHD |
Alvarez et al. (2008) |
ELISPOT |
HLA-A02.01-restricted transialidase peptides |
24 h |
IFN-γ+ lymphocytes in asymptomatic > CCHD |
Fiuza et al. (2009) |
FACS intracellular cytokine |
Epimastigote lysate (CL strain) |
22 h |
CD4CD45ROhighIFN-γ+ in asymptomatic = CCHD CD4CD45ROhighIL-10+ in asymptomatic = CCHD CD8CD45RAhighIFN-γ+ in asymptomatic = CCHD |
Lorena et al. (2010) |
FACS intracellular cytokine |
Recombinant CRA/FRA proteins |
24 h |
CRA/FRA: CD4+IFN-γ+ in asymptomatic = CCHD CD4+IL-10+ in asymptomatic = CCHD CRA: CD8+IFN-γ+ in CCHD > asymptomatic CD8+IL-10+ in asymptomatic = CCHD |
Lasso et al. (2010) |
FACS tetramer/intracellular cytokine |
HLA-A02.01-restricted K1 peptide |
12 h |
CD8+IFN-γ+ cells in asymptomatic = CCHD |
Laucella et al. (2004) |
ELISPOT |
Amastigote enriched lysate (Brazil strain) |
24 h |
IFN-γ+ cells in asymptomatic > CCHD |
Albareda et al. (2009) |
FACS intracellular cytokine |
Amastigote enriched lysate (Brazil strain) |
24 h |
CD4+IFN-γ+ in asymptomatic > CCHD |
Marañón et al. (2011) |
Bio-Plex with PBMC culture supernatants |
HLA-A02.01-restricted HSP-70 peptides |
30 h |
IFN-γ levels in asymptomatic = CCHD |
Egui et al. (2012) |
Bio-Plex with PBMC culture supernatants |
HLA-A02.01-restricted PFR2/PFR3 peptides |
30 h |
IFN-γ levels in asymptomatic > CCHD |
Gomes et al. (2012) |
FACS intracellular cytokine |
Live T. cruzi (CL strain) |
6 h |
IFN-γ+ lymphocytes in asymptomatic > CCHD IL-10-γ+ lymphocytes in asymptomatic > CCHD |
Longhi et al. (2014) |
Milli-Plex with PBMC culture supernatants |
Recombinant P2β/CPO proteins |
1-6 days |
IL-10 levels increased in CCHDa
|
Longhi et al. (2014) |
Milli-Plex with PBMC culture supernatants |
Epimastigote lysate (CL Brener, DTU TcVI) |
1-6 days |
IFN-γ and IL-10 levels increased in CCHDa
|