SciELO - Scientific Electronic Library Online

 
vol.110 número4First report of autochthonous transmission of Zika virus in BrazilUsing immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Memórias do Instituto Oswaldo Cruz

versão impressa ISSN 0074-0276versão On-line ISSN 1678-8060

Resumo

BICKERSMITH, Sara A et al. A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivaxandPlasmodium falciparum infection in field-collected anophelines. Mem. Inst. Oswaldo Cruz [online]. 2015, vol.110, n.4, pp.573-576.  Epub 29-Maio-2015. ISSN 0074-0276.  http://dx.doi.org/10.1590/0074-02760150031.

We describe a simple method for detection of Plasmodium vivaxand Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed withPlasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochromeb-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.

Palavras-chave : Anopheles; Plasmodium; TaqMan; real-time PCR.

        · texto em Inglês     · Inglês ( pdf )